|
Project summary:
The two principal objectives of this proposed project are: to assess whether 13C NMR spectroscopy can be effectively used to characterize the changes (or lack of change) in tissue chemistry during decomposition of Eucalyptus obliqua in the Warra ecosystem; and to build an NMR data set that is, at a minimum, suitable for use in developing a research grant proposal for a more comprehensive investigation of the chemodynamics of decomposition in the Warra and Hubbard Brook forests.We will use the results of experiments to determine an optimal contact time for the CPMAS analyses and to assess the quantitative efficiency of the CPMAS method for these samples.
Methodology: Samples of E. obliqua wood in varying stages of decay have been collected from the Warra LTER site. The samples have been classified visually according to decay class and type. We will select 50 of these samples which represent the range of decay classes and types. For the most common decay classes, we will select 5-7 replicate samples in order to assess the within-class variation in tissue chemistry. We will determine key spin properties of the decomposing E. obliqua tissues by carrying out variable contact time (VCT) and variable spin lock (VSL) experiments on three samples representing a range of degree of decomposition.
Datasets: None available.
Publications: None available.
|
